LEADER 00000cam  2200517 i 4500 
001    on1322488484 
003    OCoLC 
005    20231026032216.0 
006    m     o  d         
007    cr cn||||||||| 
008    220602s2022    nyua    ob    001 0 eng   
016 7  9918538123906676|2DNLM 
020    9781071621158|q(electronic bk.) 
020    1071621157|q(electronic bk.) 
020    |z9781071621141|q(print) 
024 8  10.1007/978-1-0716-2115-8|2doi 
035    (OCoLC)1322488484 
040    NLM|beng|erda|epn|cNLM|dOCLCF|dGW5XE 
042    pcc 
049    CKEA 
050  4 QR184 
082 04 571.9/648|223/eng/20220602 
130 0  Immunogenetics (Langerak) 
245 10 Immunogenetics :|bmethods and protocols /|cedited by Anton
       W. Langerak. 
264  1 New York, NY :|bHumana Press,|c[2022] 
300    1 online resource (1 PDF file (xvi, 607 pages)) :
       |billustrations (some color). 
336    text|btxt|2rdacontent 
337    computer|bc|2rdamedia 
338    online resource|bcr|2rdacarrier 
490 1  Methods in molecular biology,|x1940-6029 ;|v2453 
490 1  Springer protocols 
504    Includes bibliographical references and index. 
505 0  The Advent of Precision Immunology: Immunogenetics at the 
       Center of Immune Cell Analysis in Health and Disease -- 
       Next-Generation Sequencing-Based Clonality Detection of 
       Immunoglobulin Gene Rearrangements in B-Cell Lymphoma -- 
       One-Step Next Generation Sequencing of Immunoglobulin and 
       T-Cell Receptor Gene Recombinations for MRD Marker 
       Identification in Acute Lymphoblastic Leukemia -- 
       Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis
       Using RNA-Seq -- Minimal Residual Disease Analysis by 
       Monitoring Immunoglobulin and T-Cell Receptor Gene 
       Rearrangements by Quantitative PCR and Droplet Digital PCR
       -- Quality Control for IG/TR Marker Identification and MRD
       Analysis -- cfDNA-Based NGS IG Analysis in Lymphoma -- 
       Targeted Locus Amplification as Marker Screening Approach 
       to Detect Immunoglobulin (IG) Translocations in B-Cell Non
       -Hodgkin Lymphomas -- Immunoglobulin/T-Cell Receptor 
       Capture Strategy for Comprehensive Immunogenetics -- 
       Immunoglobulin Gene Mutational Status Assessment by Next 
       Generation Sequencing in Chronic Lymphocytic Leukemia -- 
       NGS-Based B-Cell Receptor Repertoire Analysis in the 
       Context of Inborn Errors of Immunity -- Generic Multiplex 
       Digital PCR for Accurate Quantification of T-Cells in Copy
       Number Stable and Unstable DNA Samples -- Gene Engineering
       T-Cells with T-Cell Receptor for Adoptive Therapy -- 
       Combined Analysis of Transcriptome and T-Cell Receptor 
       Alpha Beta (TRA/TRB) Repertoire in Paucicellular Samples 
       at the Single Cell Level -- AIRR Community Guide to 
       Planning and Performing AIRR-Seq Experiments -- Adaptive 
       Immune Receptor Repertoires (AIRR) Community Guide to TR 
       and IG Gene Annotation -- Adaptive Immune Receptor 
       Repertoires (AIRR) Community Guide to Repertoire Analysis 
       -- Bulk gDNA Sequencing of Antibody Heavy Chain Gene 
       Rearrangements for Detection and Analysis of B-Cell Clone 
       Distribution: A Method by the AIRR Community -- Bulk 
       Sequencing from mRNA with UMI for Evaluation of B-Cell 
       Isotype and Clonal Evolution: A Method by the AIRR 
       Community -- Single Cell Analysis and Tracking of Antigen-
       Specific T-Cells: Integrating Paired-Chain AIRR-Seq and 
       Transcriptome Sequencing: A Method by the AIRR Community -
       - Quality Control: Chain Pairing Precision and Monitoring 
       of Cross-Sample Contamination: A Method by the AIRR 
       Community -- Immune Repertoire Analysis on High-
       Performance Computing Using VDJServer V1: A Method by the 
       AIRR Community -- Data Sharing and Re-Use: A Method by the
       AIRR Community -- IMGT<U+fffd> Immunoinformatics Tools for
       Standardized V-DOMAIN Analysis -- IMGT/3Dstructure-DB: T-
       Cell Receptor TR Paratope and Peptide/Major 
       Histocompatibility pMH Contact Sites and Epitope -- ARResT
       /Interrogate Immunoprofiling Platform: Concepts, Workflows,
       and Insights -- Purpose-Built Immunoinformatics for BcR IG
       /TR Repertoire Data Analysis. 
506 0  Open access.|5GW5XE 
520    This open access book explores techniques for working in 
       the field of immunogenetics, i.e. fundamental and 
       translational research into the adaptive immune receptor 
       repertoire. Many chapters are dedicated to lab protocols, 
       bioinformatics, and immunoinformatics analysis of high-
       resolution immunome analysis, exemplified by numerous 
       applications. Additionally, the newest technological 
       variations on these protocols are discussed, including non
       -amplicon, single-cell, and cell-free strategies. Written 
       for the highly successful Methods in Molecular Biology 
       series, chapters include introductions to their respective
       topics, lists of the necessary materials and reagents, 
       step-by-step, readily reproducible laboratory protocols, 
       and tips on troubleshooting and avoiding known pitfalls. 
       Authoritative and practical, Immunogenetics: Methods and 
       Protocols covers a broad spectrum of methodologies for 
       applications in research and clinical diagnostics to 
       illustrate the impact that immunogenetics has achieved and
       will further expand in all fields of medicine, from 
       infection and (auto)immunity, to vaccination, to lymphoid 
       malignancy and tumor immunity. 
588 0  Description based on online resource; title from PDF title
       page (SpringerLink, viewed June 2, 2022). 
590    Springer Nature|bSpringer Nature Open Access eBooks 
650  0 Immunogenetics. 
650  7 Immunogenetics.|2fast|0(OCoLC)fst00967949 
650 12 Immunogenetic Phenomena. 
655  2 Laboratory Manual. 
700 1  Langerak, Anton W.|eeditor. 
830  0 Methods in molecular biology (Clifton, N.J.) ;|vv. 2453.
       |x1064-3745 
830  0 Springer protocols (Series)|x1949-2448 
914    on1322488484 
994    C0|bCKE