| Description |
1 online resource |
| Bibliography |
Includes bibliographical references and index. |
| Contents |
Front Cover; Ecometabolomics: Metabolic Fluxes Versus Environmental Stoichiometry; Copyright; Dedication; Contents; Preface; Acknowledgements; About the Book; Chapter 1: Introducing Metabolomics; 1.1. Introduction; 1.2. Non-Targeted Identification and Quantification of All Metabolites; 1.2.1. Quality Control Samples; Quality Control Procedures; 1.2.2. Steps Involved in Non-Targeted Metabolomics; Selection of Sample; Sample Treatment and Analytical Conditions; Analytical Method Validation; 1.3. Quantitative Detection of Metabolites; 1.4. Identification and Quantification of Metabolites |
|
1.5. Targeted Metabolite Analysis 1.5.1. Metabolic Flux Analysis; 1.6. Metabolite Fingerprinting; 1.6.1. Metabolite Profiling of Plant Tissues Using NMR; 1.7. Metabolite Footprinting; 1.8. Conclusion; References; Further Reading; Chapter 2: An Integrated Approach to Plant Biology via Multi-Analogous Methods; 2.1. Introduction; 2.2. Transcript Profiling; 2.2.1. Library Preparation from RNA Samples; 2.2.2. MicroRNA Profiling; 2.3. Microarray-Based Approaches; 2.3.1. Comparative Genomic Hybridization to Microarrays; 2.3.2. The International HapMap Project; 2.3.3. SNP Genotyping Arrays |
|
2.3.4. SNP Genotyping Array Studies 2.4. Sequencing-Based Approaches; 2.4.1. Advantages of RNA-Seq Technology; 2.4.2. Challenges for RNA-Seq Library Construction; 2.5. Differential-Display-Based Approaches; 2.5.1. Amplified Fragment Length Polymorphism; 2.5.2. Advantages of AFLP Analysis; 2.6. Protein Analysis; 2.6.1. Two-Dimensional Gel Electrophoresis; 2.6.2. Capillary IEF; Principle of IEF; Instrumentation of Capillary Iso-electro Focusing; Applications of Capillary IEF; 2.7. Metabolite Analysis; 2.7.1. Metabolic Fingerprinting; 2.7.2. Metabolite Profiling; 2.8. Analytical Techniques |
|
2.8.1. Gas Chromatography Coupled With Mass Spectrometry 2.8.2. Nuclear Magnetic Resonance; 2.8.3. Mass Spectrometry; 2.8.4. Liquid Chromatography-Mass Spectrometry; 2.8.5. Capillary Electrophoresis-Mass Spectrometry; 2.8.6. Other Technologies; 2.9. Pattern Recognition; 2.10. Multivariate Statistics Techniques; 2.10.1. Principal Component Analysis; 2.10.2. Partial Least Squares; 2.10.3. Orthogonal Projection to Latent Structures; 2.11. Bioinformatics for Metabolomics; 2.12. Conclusions; References; Further Reading; Chapter 3: Metabolomics Studies of Stress in Plants; 3.1. Introduction |
|
3.2. Temperature Stress 3.2.1. Low-Temperature Response; 3.2.2. High-Temperature Stress Response; 3.3. Water and Salt Stress; 3.4. Sulfur and Phosphorus Stress; 3.5. Oxidative Stress; 3.6. Heavy Metal Stress; 3.7. Metabolomic Analysis of Plant-Stress Interactions; 3.8. Metabolic Network Implicated in Stress Signaling; 3.8.1. Functional Significance of ABA in Metabolic Adjustments; 3.8.2. Role of Proline Accumulation in Signaling; 3.8.3. Transcription Factors Regulating Metabolite Levels; 3.9. Conclusion; References; Further Reading; Chapter 4: Climatic Change and Metabolome Fluxes |
| Summary |
'Ecometabolomics: Metabolic Fluxes versus Environmental Stoichiometry' focuses on the interaction between plants -- particularly plants that have vigorous secondary metabolites -- and the environment. The book offers a comprehensive overview of the responses of the metabolome of organisms to biotic and abiotic environmental changes. It includes an introduction to metabolomics, summaries of metabolomic techniques and applications, studies of stress in plants, and insights into challenges. This is a must-have reference for plant biologists, plant biochemists, plant ecologists and phytochemists researching the interface between plants and the environment using metabolomics. Key Features: Provides an in-depth overview of the basics of the discipline, including non-targeted analysis and quantification of plant metabolites; Outlines the applications of various analytical techniques in comprehending the total metabolome of the organism; Covers both NMR and MS-based approaches. -- Provided by publisher. |
| Note |
Online resource; title from digital title page (viewed on April 16, 2019). |
| Subject |
Plant ecology.
|
|
Metabolomics. (DNLM)D055432
|
|
SCIENCE -- Life Sciences -- Botany.
|
|
Plant ecology. (OCoLC)fst01065415
|
| Added Author |
Ahmad, Parvaiz, author.
|
| Other Form: |
Print version: Jan, Sumira. Ecometabolomics. London, United Kingdom ; San Diego, CA, United States : Academic Press is an imprint of Elsevier, [2019] 0128148721 9780128148723 (OCoLC)1082186059 |
| ISBN |
9780128148730 (electronic book) |
|
012814873X (electronic book) |
|
0128148721 |
|
9780128148723 |
|
